Experiment:

Objective: To look at differentially expressed genes in resistant and sensitive cultivars of soybean, during the invasion of the soybean cyst nematode (SCN) for a duration of 48 hours.

Protocols:

•  Amplification
•  Array Printing
•  Probe Preparation and Hybridization

Data: 

The microarray scans and extracted data for experiment SCNT6 are provided.  

Data Analysis:

The data from this experiment was analyzed using two image analysis software packages, one a freeware from the Eisen Laboratory called ScanAlyze, and the other a commercially available package called SPOT from Australia's Commonwealth Scientific and Industrial Research Organization (CSIRO), Mathematical and Information Sciences division. SPOT has the advantage over ScanAlyze in that it automatically conforms to the individual arrayed spots dimensions in the image, thereby giving a more accurate reading in our opinion The final results obtained from both were somewhat similar however.

Below are links to pages that contain the results of different analysis methodologies that were applied to both data sets:

• ScanAlyze Data Analysis
  
  
• SPOT Data Analysis