DNA microarray experiments are performed at the Matthew's laboratory to identify resistance genes in soybean against a dangerous pest known as the soybean cyst nematode (SCN) that causes approximately $1.5 billion is losses to the US economy annually.

A number of such experiments have been performed, they are listed below along with the goal of each.

• SCNT5: This is a preliminary experiment and shows protocols, gel images of amplifications of clone inserts that were printed as features on the microarray. Peking was inoculated for 48 hr with SCN (P+) and compared with uninoculated Peking (P-), Kent inoculated with SCN (K+) and uninoculated Kent (K-) roots. Images of the microarray slides are provided.

• SCNT6: Hybridization of probes from soybean cultivar Kent roots inoculated with SCN for 48 hr compared with uninoculated Kent roots. Hybridization of probes from Peking roots inoculated with SCN for 48 hrs compared with uninoculated Kent roots. Hybridization of probes from Peking roots inoculated with SCN for 48 hrs compared with uninoculated Peking roots. Preliminary experiment using approximately 1300 cDNA inserts printed in quadruplicate. 

• SCNT7: This is our 1st time series experiment involving probes from soybean cultivar Kent and Peking roots inoculated with SCN for 6 hr., 12 hr., 24 hr., 48 hr., 4 days, 6 days and 8 days, hybridized to probes from Kent and Peking uninoculated respectively. Controls include self-self hybridized slides of Peking and Kent.  Experiment involved the use of approximately 3816 cDNA clones printed in triplicate.