Collaborator: Dr. Halina Knap
Faculty of Genetics Program
Department of Crop and Soil Environmental Science
Department of Biological Sciences
Clemson University
Clemson, S.C. 29634-0359
Voice: 864-656-3523
Fax:   864-656-3443
hskrpsk@clemson.edu

    Dr. Knap’s laboratory collaborates with the Matthews Laboratory on two projects. The first project is to clone genes conferring resistance to the soybean cyst nematode by map position. This requires the use of Bacterial Artificial Chromosomes (BACs) and other sophisticated molecular and genetic tools.  The second project is to identify and determine the function of genes involved in the resistance response of soybean to the soybean cyst nematode.  This will lead to the use of these genes in new SCN-resistant varieties for farmers. 

    The Knap laboratory constructed two subtractive cDNA libraries from roots of PI437654, a resistant soybean plant introduction.   A forward subtractive library was made using root mRNA from roots 10 hrs after infection by SCN as tester and mRNA from non-infected roots as driver.  From this library 444 clones were sequenced as ESTs.  The second library was constructed as a reverse library in which the non-infected root mRNA was used as tester and mRNA from the infected roots was used as driver. 332 clones were sequenced as ESTs from this library.  The expression of the genes represented by these clones are being studied using microarrays to identify which genes are increased in amount of expression during the resistance response.

For collaborators of this project only:

Knap clones (SSH clones)

Search Knap clones

Microarray data